How to Submit Raw Reads

Introduction

To submit raw read sequencing data to ENA you must also provide some metadata to describe your sequencing project. This helps make your data re-useable and searchable.

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Within ENA, raw reads are represented as ‘run’ and ‘experiment’ submission objects.

The run submission holds information about the raw read files generated in a run of sequencing.

The experiment submission holds metadata that describe the methods used to sequence the sample.

If you are not yet familiar with the metadata model, please see here for some more information.

As a raw read submission references ENA sample and study objects, you must submit these before your submit your read data. See below for information on how to register a study within ENA to describe your overall research project and samples with information on the biological data that was sequenced:

Accessions

Once the raw reads are registered, Webin will report two unique accession numbers for each read submission. The first starts with ERR and is called the Run accession. The other starts with ERX and is called the Experiment accession.

Always make a note of any accessions you receive as these are the unique identifiers for each of your submissions to ENA.

Submission Options

Reads can be submitted in one of three ways. For an overview of these, please see the General Guide on Submitting to ENA.